Lipase Activity of Mycoplasma

Lipase Activity of Mycoplasma.

Enzymatic activity of lipase

The knowledge of the digestive system of bullfrogs is an important step for the determination of their nutritional requirements throughout growth phases. With the objective of evaluating the enzymatic activity of lipase in the intestinal content of bullfrogs (Rana catesbeiana Shaw, 1802), 100 animals with median weight of 3.6 g were distributed in stalls under controlled temperature and photope...

Determination of lipase activity.

Heretofore most methods for the determination of lipase activity have employed emulsions of fatty acid esters in water (1). These emulsions have been stabilized by use of such agents as gum acacia. The disadvantages of such methods are that (1) the emulsions break up to a considerable and variable extent during the course of the incubation, and (2) the enzyme is presumed to be in the aqueous ph...

Assaying Arabidopsis lipase activity

A low lipase activity from a crude extract of Arabidopsis seedlings was assayed using three sensitive methods (radiolabelled triacylglycerols, commercial resorufin ester and triacylglycerols containing the naturally fluorescent parinaric acid as substrates). The specific activity of the extract was found to be similar using the three methods. However, the plant lipase activity measured using th...

Neuraminidase activity in Mycoplasma gallisepticum.

The whole viable Mycoplasma gallisepticum (strain TT) organisms were found to possess neuraminidase activity with a pH optimum of 5.8 on substrates such as human transferrin, human alpha(1)-glycoprotein, and rabbit serum. The enzyme operated optimally at pH 4.5 when N-acetylneuraminyl-lactose was used as the test substrate.